SIMPLE METHOD FOR THE PREPARATION OF HAPTOGLOBIN

  • 1 January 1979
    • journal article
    • research article
    • Vol. 38  (4) , 669-672
Abstract
A method permitting the isolation of haptoglobin 2-2 from human serum or plasma is presented. The haptoglobin is adsorbed to DEAE-cellulose at pH 5.1 by batching. The loaded cellulose is put into a column and the haptoglobin eluted by a 0.1-0.15 M acetate buffer. The last step is gel chromatography on Sephadex G-200. Disc electrophoresis and immunoelectrophoresis were used to test the purity. Acid .alpha.1-glycoprotein can be obtained as a by-product.

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