Computational Analysis of the Polymorphic Membrane Protein Superfamily ofChlamydia trachomatisandChlamydia pneumoniae
- 1 January 1999
- journal article
- research article
- Published by Mary Ann Liebert Inc in Microbial & Comparative Genomics
- Vol. 4 (3) , 187-201
- https://doi.org/10.1089/omi.1.1999.4.187
Abstract
Whole sequence genome analysis is invaluable in providing complete profiles of related proteins and gene families. The genome sequences of the obligate intracellular bacteria Chlamydia trachomatis and Chlamydia pneumoniae both encode proteins with similarity to several 90-kDa Chlamydia psittaci proteins. These proteins are members of a large superfamily, C. trachomatis with 9 members and C. pneumoniae with 21 members. All polymorphic membrane proteins (Pmp) are heterogeneous, both in amino acid sequence and in predicted size. Most proteins have apparent signal peptide leader sequences and hence are predicted to be localized to the outer membrane. The unifying features of all proteins are the conserved amino acid motifs GGAI and FXXN repeated in the N-terminal half of each protein. In both genomes, the pmp genes are clustered at various locations on the chromosome. Phylogenetic analysis suggests six related families, each with at least one C. trachomatis and one C. pneumoniae orthologue. One of these families has seen prolific expansion in C. pneumoniae, resulting in 13 protein paralogues. The maintenance of orthologues from each species suggests specific functions for the proteins in chlamydial biology.Keywords
This publication has 49 references indexed in Scilit:
- Immunoelectron microscopic localisation of the OMP90 family on the outer membrane surface ofChlamydia psittaciFEMS Microbiology Letters, 1998
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- Two‐dimensional electrophoretic analysis of the protein family at 90 kDa of abortifacientChlamydia psittaciElectrophoresis, 1997
- Promoter strength influences phase variation of neisserial opa genesMolecular Microbiology, 1997
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Antigen diversity in the bacterium B. hermsii through “Somatic” mutations in rearranged vmp genesCell, 1994
- Carboxy-terminal phenylalanine is essential for the correct assembly of a bacterial outer membrane proteinJournal of Molecular Biology, 1991
- Basic local alignment search toolJournal of Molecular Biology, 1990
- A simple method for displaying the hydropathic character of a proteinJournal of Molecular Biology, 1982
- Conformational properties of amino acid residues in globular proteinsJournal of Molecular Biology, 1976