Naturally Occurring Anti- Escherichia coli Protein Antibodies in the Sera of Healthy Humans Cause Analytical Interference in a Recombinant Nucleocapsid Protein-Based Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Severe Acute Respiratory Syndrome

1 January 2007

journal article
Published by American Society for Microbiology in Clinical and Vaccine Immunology

Vol. 14 (1) , 99-101
https://doi.org/10.1128/cvi.00136-06

Abstract

We reported the analytical interference of anti- Escherichia coli protein (EP) antibodies in human sera and residual EP in a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay as a possible source of false positives in severe acute respiratory syndrome serodiagnosis. The rate of false positives was significantly reduced by adding mouse anti-EP antiserum in the blocking step.

Keywords

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