High Pressure Liquid Chromatographic Determination of Cholesterol in Foods
- 1 January 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 64 (1) , 54-57
- https://doi.org/10.1093/jaoac/64.1.54
Abstract
A high pressure liquid chromatographic method that uses the benzoate ester of cholesterol has been developed for the measurement of cholesterol in foods. A 300 × 3.9 mm id μBondapak C18 column and a variable wavelength ultraviolet detector are used with a 100% methanol mobile phase. Amounts as low as 10 ng cholesterol benzoate can be detected. The results from the analyses of a variety of foods are given and compared with results from gas-liquid chromatographic analyses. The comparison of the 2 chromatographic techniques is favorable.This publication has 0 references indexed in Scilit: