The Drosophila nucleosome remodeling factor NURF is required for Ecdysteroid signaling and metamorphosis
Open Access
- 1 November 2005
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 19 (21) , 2540-2545
- https://doi.org/10.1101/gad.1342605
Abstract
Drosophila NURF is an ISWI-containing ATP-dependent chromatin remodeling complex that regulates transcription by catalyzing nucleosome sliding. To determine in vivo gene targets of NURF, we performed whole genome expression analysis on mutants lacking the NURF-specific subunit NURF301. Strikingly, a large set of ecdysone-responsive targets is included among several hundred NURF-regulated genes. Null Nurf301 mutants do not undergo larval to pupal metamorphosis, and also enhance dominant-negative mutations in ecdysone receptor. Moreover, purified NURF binds EcR in an ecdysone-dependent manner, suggesting it is a direct effector of nuclear receptor activity. The conservation of NURF in mammals has broad implications for steroid signaling.Keywords
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