Detection of glycoprotein IIb and IIIa by monoclonal antibodies

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Abstract
Murine monoclonal antibodies were produced against human platelet membranes and screened on platelets by a 125 protein A radioimmunoassay. Several clones produced platelet specific antibodies as they showed no reaction with peripheral blood lymphocytes, neutrophils, bone marrow (excluding megakaryocytes) or several cell lines. Two antibodies (designated anti-HuPl-m1a and anti-HuPl-m1b) were of particular interest in that although platelet specific they were non-reactive with platelets from a thrombasthenic patient. In functional assays these 2 antibodies could specifically inhibit ADP and collagen induced aggregation of platelets and release of ATP, retard platelet aggregation and ATP release induced by epinephrine, and inhibit ADP induced platelet fibrinogen binding. These 2 antibodies appear to recognize glycoproteins IIb and IIIa as analysis by SDS-PAGE [sodium dodecyl sulfate polyacrylamide gel electrophoresis] using radiolabeled membranes revealed a 2 chain structure of MW 112,000 and 122,000 daltons when run after reduction and 87,000 and 140,000 daltons non-reduced.