Thermally induced biosynthesis of 2'-O-methylguanosine in tRNA from an extreme thermophile, Thermus thermophilus HB27.
- 1 April 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (4) , 1922-1926
- https://doi.org/10.1073/pnas.77.4.1922
Abstract
The contents of 29-O-methylguanosine and 1-methyladenosine in unfractionated tRNA obtained from Thermus thermophilus HB27 were found to increase significantly when the bacterium was grown at a higher temperature (80 degrees C). S-Adenosyl-L-methionine-dependent tRNA (guanosine-29)-methyltransferase (EC 2.1.1.34) and tRNA (adenine-1)-methyltransferase (EC 2.1.1.36) were detected in a cell-free extract of the thermophile, and both of them were partially purified. tRNA (guanosine-29)-methyltransferase specifically catalyzed the methylation of the guanylate residue at position 19 from the 59 end of Escherichia coli tRNAMetf. The amounts of these methyltransferases in the cells and their thermal characteristics seemed to be independent of the growth temperature of the bacterial cells from which the enzymes were extracted. It was inferred that the temperature dependence of the methylation process in vivo is accounted for, not by temperature dependence of enzyme formation, but by that of the enzyme activity.Keywords
This publication has 22 references indexed in Scilit:
- The structure of the anticodon loop of tRNAPhe from yeast as deduced from spectroscopic studies on oligonucleotidesBiopolymers, 1975
- Replacement of ribothymidine by 5‐methyl‐2‐thiouridine in sequence GTψC in tRNA of an extreme thermophileFEBS Letters, 1974
- Biosynthesis of 5-methylaminomethyl-2-thiouridylate. I. Isolation of a new tRNA-methylase specific for 5-methylaminomethyl-2-thiouridylateBiochemical and Biophysical Research Communications, 1973
- The effect of growth temperatures on the in vivo ribose methylation of Bacillus stearothermophilus transfer RNAArchives of Biochemistry and Biophysics, 1973
- Minor components in transfer RNA: their characterization, location, and function.1972
- Primary sequence of tRNA1Val from Escherichia coli. B. I. Oligonucleotide sequences of digests of Escherichia coli tRNA1val with RNase T1 and pancreatic RNaseBiochemistry, 1971
- Thermal stability of ribosomes and RNA from Thermus aquaticusBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1970
- The Nucleotide Sequence of Methionine Transfer RNAMEuropean Journal of Biochemistry, 1970
- Recovery of transfer RNA functions by combining fragmented Escherichia coli formylmethionine transfer RNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
- The in vitro synthesis of 2′-omethylguanosine and 2-methylthio 6N (γ,γ, dimethylallyl) adenosine in transfer RNA of Escherichia coliBiochemical and Biophysical Research Communications, 1969