Primary structure of neutral oligosaccharides derived from respiratory‐mucus glycoproteins of a patient suffering from bronchiectasis, determined by combination of 500‐MHz 1H‐NMR spectroscopy and quantitative sugar analysis

Open Access
Publisher Website
Google Scholar
Abstract
Carbohydrate chains of respiratory‐mucus glycopeptides from a patient (blood group O) suffering from bronchiectasis with a Kartagener's syndrome have been released by alkaline borohydride treatment. Application of high‐performance liquid chromatography using subsequently two silica columns, one bonded with aminopropyl groups and the other with octadecyl groups, afforded 39 neutral fractions; 35 oligosaccharide‐alditol structures have been characterized by employing 500‐MHz 1H‐NMR spectroscopy in conjunction with sugar analysis. Here, 16 oligosaccharide structures, possessing a core consisting of Galβ(1→3)GalNAc‐ol with or without branching through a GlcNAc residue linked β(1→6) to the GalNAc residue (core type 2 or core type 1, respectively), are described. Ten oligosaccharide‐alditols with these types of cores (2, 3, 10a, 14, 7, 11a, 15a, 16a, 12 and 16c) have been identified previously in human bronchial mucins of patients suffering from cystic fibrosis [Lamblin, G., Boersma, A., Lhermitte, M., Roussel, P., Mutsaers, J. H. G. M., Van Halbeek, H. and Vliegenthart, J. F. G. (1984) Eur. J. Biochem. 143, 227–236]. Of the remaining six compounds, one is a partial structure of oligosaccharides previously described: The structures 17a and 20 contain the Y determinant, i.e., Fucα(1→2)Galβ(1→4)[Fucα(1→3)]GlcNAcβ(1→). High‐resolution 1H‐NMR spectroscopy is able to distinguish whether the Y determinant is β(1→3) or β(1→6) linked in such oligosaccharide‐alditols.

This publication has 17 references indexed in Scilit: