EFFECT OF p‐BROMOPHENACYL BROMIDE, AN INHIBITOR OF PHOSPHOLIPASE A2, ON ARACHIDONIC ACID RELEASE AND PROSTAGLANDIN SYNTHESIS BY THE GUINEA‐PIG UTERUS in vitro
Open Access
- 1 January 1977
- journal article
- Published by Wiley in British Journal of Pharmacology
- Vol. 59 (1) , 107-113
- https://doi.org/10.1111/j.1476-5381.1977.tb06983.x
Abstract
1 The synthesis of prostaglandins F2α and E2 by guinea-pig uterine homogenates was inhibited by p-bromophenacyl bromide (PBPAB), an inhibitor of phospholipase A2. 2 Metabolism of prostaglandin F2α by uterine homogenates was undetectable; this was not affected by PBPAB. 3 There was no significant difference between the amounts of arachidonic acid released from uterine homogenates on days 7 and 15 of the oestrous cycle. Small amounts of dihomo-γ-linolenic acid were detected in the homogenates. 4 The release of arachidonic acid from uterine homogenates was greatly inhibited by PBPAB. 5 Addition of exogenous arachidonic acid to uterine homogenates did not overcome the inhibition of uterine prostaglandin F2α synthesis produced by PBPAB. 6 It is concluded that PBPAB inhibits both the release of arachidonic acid from the guinea-pig uterus and its subsequent conversion into prostaglandins.Keywords
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