Activation of membrane phospholipase C by vasopressin
- 3 February 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 196 (1) , 155-159
- https://doi.org/10.1016/0014-5793(86)80232-0
Abstract
Vasopressin stimulates the liberation of labelled inositol phosphate in partially purified plasma membranes prepared from myo-[3H]inositol prelabelled WRK1 cells. This stimulatory effect was very rapid (165% stimulation of inositol trisphosphate accumulation after a 10 s incubation period in the presence of 1 μM vasopressin), concentration dependent (EC50= 12 nM) and was abolished by an antagonist of the vasopressor response to vasopressin. GTP, even at high concentrations (0.1 mM), did not increase inositol phosphate release: it was found to be absolutely necessary for hormonal stimulation of phospholipase C activity. Non-hydrolysable analogues of GTP may also stimulate this enzyme activity.Keywords
This publication has 11 references indexed in Scilit:
- Regulation of a liver plasma membrane phosphoinositide phosphodiesterase by guanine nucleotides and calciumFEBS Letters, 1985
- 5-Hydroxytryptamine stimulates inositol phosphate production in a cell-free system from blowfly salivary glands. Evidence for a role of GTP in coupling receptor activation to phosphoinositide breakdown.Journal of Biological Chemistry, 1985
- Inositol trisphosphate, a novel second messenger in cellular signal transductionNature, 1984
- Carboxy terminus of vasopressin required for activity but not bindingNature, 1984
- Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphateNature, 1983
- Involvement of guanine nucleotide-binding protein in the gating of Ca2+ by receptorsNature, 1983
- Rapid breakdown of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate in rat hepatocytes stimulated by vasopressin and other Ca2+-mobilizing hormonesBiochemical Journal, 1983
- Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositidesBiochemical Journal, 1983
- DIRECT STIMULATION OF PHOSPHATIDYLINOSITOL DEGRADATION BY ADDITION OF VASOPRESSIN TO PURIFIED RAT LIVER PLASMA MEMBRANESEndocrinology, 1982
- (3H)-Vasopressin Binding to Isolated Rat Hepatocytes and Liver Membranes: Regulation by GTP and Relation to Glycogen Phosphorylase ActivationJournal of Receptor Research, 1980