Infinite Average Lifetime of an Unstable Bright State in the Green Fluorescent Protein
- 25 August 2005
- journal article
- Published by American Physical Society (APS) in Physical Review Letters
- Vol. 95 (9) , 090602
- https://doi.org/10.1103/physrevlett.95.090602
Abstract
The time evolution of the fluorescence intensity emitted by well-defined ensembles of green fluorescent proteins has been studied by using a standard confocal microscope. In contrast with previous results obtained in single-molecule experiments, the photobleaching of the ensemble is well described by a model based on Lévy statistics. By assuming the presence of thermally activated barriers, this simple model allows us to obtain information about their height distribution.Keywords
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