Diamide blocks H+ conductance in mitochondrial H+‐ATPase by oxidizing FB dithiol
- 11 November 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 192 (1) , 83-87
- https://doi.org/10.1016/0014-5793(85)80048-x
Abstract
Effects of diamide on proton conductance of electron transport particles (ETPH), purified H+‐ATPase (F1–F0), F0 of the H+‐ATPase from beef heart mitochondria and binding of cadmium (109Cd) to the H+‐ATPase have been examined in the present paper. When ETPH and purified H+‐ATPase are treated with 1 mM diamide, ATP‐dependent generation of membrane potential, monitored by the absorbance change produced by the redistribution of oxonol VI, is consistantly inhibited. Diamide also blocks passive H+ conductance driven by a K+ diffusion potential in the membrane sector, F0 of H+‐ATPase. Furthermore, diamide treatment drastically reduces the binding of 109Cd2+ to H+‐ATPase, showing competition for the FB dithiol group.Keywords
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