ERYTHROCYTE PRESERVATION. II. A STUDY OF EXTRA-ERYTHROCYTE FACTORS IN THE STORAGE OF BLOOD IN ACID-CITRATE-DEXTROSE 1

Abstract
The role of the extra-erythrocytic environment in the production of the storage lesion in the erythrocyte was investigated. Damage to the stored erythrocyte was measured by osmotic fragility, phosphate partition, and viability after transfusion. There was no evidence of a deleterious effect of citrate, plasma, leukocytes, reticulocytes, or hemolysates. Throughout there was a notable consistency in the appearance of the chemical changes measured and in loss of viability of the erythrocyte. It would appear that when blood is stored at 0 to 4oC in acid citrate dextrose the storage lesion in the erythrocyte bears little relation to its surrounding medium.
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