SHORT-TERM STORAGE OF HUMAN LYMPHOCYTES PRIOR TO IN VITRO STIMULATION1,2

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Abstract
In order to transport human lymphocytes long distances prior to in vitro stimulation a method of short-term storage was developed. Cells stored for 72 hr at room temperature in RPMI 1640 medium with 15% pooled allogeneic plasma retain ability to respond in vitro to stimulation with phytohemagglutinin (PHA), soluble protein antigens, and the mixed lymphocyte reaction. Storage for 2 weeks at 37 C in a 5% CO2 atmosphere was also accomplished. These lymphocytes are able to respond to PHA. Addition of fresh macrophages or macrophage culture supernatant is needed to restore response to specific antigen and irradiated allogeneic lymphocytes.

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