Characteristics of a highly thermostable neutral protease produced fromBacillus stearothermophilus
- 1 January 1991
- journal article
- Published by Springer Nature in World Journal of Microbiology and Biotechnology
- Vol. 7 (1) , 12-16
- https://doi.org/10.1007/bf02310912
Abstract
A highly thermostable neutral protease was found in culture filtrates ofBacillus stearothermophilus. The optimum reaction pH and temperature of this protease were 6.0 and 60°C, respectively, and 90% activity remained even after heat treatment at 90°C for 30 min. The protease was markedly inactivated by diisopropyl fluorophosphate, but EDTA and iodoacetic acid hardly affected it. The neutral protease therefore could be defined as a highly thermostable, neutral(-serine) protease.Keywords
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