Possible Phospholipid Precursor for Phosphatidylserine in Rat Heart

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Abstract
The incorporation of 32P‐labelled inorganic phosphate into phospholipids of rat heart slices was investigated in pulse‐chase experiments. The comparison of the specific activities of individual phospholipids suggested that phosphatidic acid can be 32P precursor for phosphatidylserine. Studies with l‐serine revealed the full calcium dependence of phosphatidylserine formation in heart homogenates. Chasing experiments with [1,2‐14C]ethanolamine and [methyl‐14C]choline proved the precursor role of phosphatidylethanolamine and phosphatidylcholine and indicated a base exchange mechanism. Among phospholipid phosphatidic acid was the most potent in stimulating phosphatidylserine formation in homogenates. The conclusion was reached that phosphatidic acid can also be regarded as precursor for phosphatidylserine.