Isolation of a Gonadotropin (PMEG) From Pregnant Mare Endometrial Cups: Comparison with PMSG

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Abstract
PMEG [pregnant mare endometrial gonadotropin] was purified and compared to pregnant mare serum gonadotropin (PMSG). Purification methodology applicable to PMSG was employed. In vivo and in vitro assays for FSH [follicle stimulating hormone] and LH [luteinizing hormone] were used to evaluate PMEG preparations. In all cases, lower activities (11-54%) were observed with PMEG compared to PMSG. Antiserum raised in rabbits against PMSG cross-reacts fully with PMEG in agar double diffusion tests. The amino acid composition of PMEG is similar to PMSG, but amino terminal group analyses show PMEG preparations to be more heterogeneous than PMSG. The carbohydrate composition of all PMEG preparations examined was considerably less (13-30%) than found in PMSG (45%).