OBSERVATIONS ON THE ISOLATION OF SALMONELLAE FROM SELENITE BROTH

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Abstract
SUMMARY: Studies of growth curves of enterobacteria in selenite broth containing different carbohydrates showed that whereas mannitol and lactose brought about a steep fall in coli‐aerogenes bacteria, mannitol improved the growth of Salm. typhi‐murium. With mixed cultures of Cit. freundii I and Salm. typhi‐murium the presence of lactose, utilizable by the former, adversely affected the viable count of the latter.Comparative studies with routine faeces specimens showed that selenite broth was an efficient selective medium with MacConkey's agar; but much better results were obtained when it was combined with deoxycholate‐citrate agar. Gentian violet introduced into selenite broth improved its selectivity for most Salmonella types when MacConkey's agar was used for final isolation.