Detection of β2‐Microglobulin in Sera from Diverse Vertebrate Species by the Use of a Heterologous Radioimmunoassay

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Abstract
The strong cross-reaction between rabbit and human .beta.2-microglobulins, as detected by goat antisera, has permitted the development of heterologous radioimmunoassays to detect homologues of .beta.2-microglobulin (.beta.2.mu.) in the sera of various vertebrate species [human, rabbit, African green monkey, horse, dog, cat, guinea pig, rat, pig, opossum, bandicoot, wallaby, cow, sheep, turkey, chicken, sand shark]. The assay based on the inhibition of the binding reaction between goat anti-rabbit .beta.2.mu. and human .beta.2.mu. divided the sera examined into 2 groups, 1 giving high levels of inhibition (.apprx. 80%), and the other lower levels (.apprx. 20%). There was no evident correlation between this division and phylogenetic classification. When the same group of sera was tested using the assay based on the inhibition of binding of rabbit .beta.2.mu. to goat anti-human .beta.2.mu., a wide range of maximal levels of inhibition was observed, with clustering of phylogenetically related species.