Significance ofahpC promoter mutations for the prediction of isoniazid resistance inMycobacterium tuberculosis
- 1 July 1998
- journal article
- Published by Springer Nature in European Journal of Clinical Microbiology & Infectious Diseases
- Vol. 17 (7) , 508-511
- https://doi.org/10.1007/bf01691135
Abstract
To determine the value ofahpC promoter mutations for the rapid prediction of isoniazid resistance, this genomic region was characterized in 50 isoniazid-resistant and 12 isoniazid-sensitiveMycobacterium tuberculosis isolates. Of the resistant isolates, 12 hadahpC promoter mutations, but only one possessed both anahpC promoter mutation and akatG codon 315 substitution, although the latter was found in the majority (54%) of the isoniazid-resistant isolates investigated. This investigation presents empirical evidence that the central portion of theahpC promoter is the most valuable genetic locus to complementkatG codon 315 characterizations in order to increase the sensitivity of molecular tests for the prediction of isoniazid resistance.Keywords
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