Procedure for the Determination of Milk Proteins by Dye Binding

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Abstract
Previously published dye-binding methods and equipment were changed. A 0.75 mm light-path flow-through cuvette was changed to fill from the bottom. One-half ml of milk was mixed with 30 ml dye solution (0.4110 g Amido-Black 10B dissolved in one 1 of 0.3 m citric-acid), centrifuged, the supernatant poured into the curvette and the optical density determined at 615 mu. The difference between the sample value and the optical density of a water blank prepared in a similar manner was used to determine the percent protein from a standard curve. The correlation coefficient between data obtained with this dye-binding procedure and the Kjeldakh procedure (N x 6.38) was 0.947.

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