Improved Media for Sporulation and Enterotoxin Production by Clostridium perfringens

1 September 1986

journal article
research article
Published by Elsevier in Journal of Food Protection

Vol. 49 (9) , 706-711
https://doi.org/10.4315/0362-028x-49.9.706

Abstract

Sporulation of 24 strains of Clostridium perfringens isolated from stools of food poisoning patients and normal controls was improved by adding sodium carbonate to Duncan-Strong (DS) sporulation medium and replacing starch with raffinose in Taniguti's AEA medium. Viable counts of heat-tolerant spores (75°C for 20 min) were 2 to 186 times greater in modified AEA medium and 2 to 169 times greater in modified DS than in DS medium. Reversed passive latex agglutination assays revealed a corresponding increase in enterotoxin titers in supernatant fluids of the 12 enterotoxigenic strains grown in modified AEA medium and in modified DS medium.

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