LIBERATION OF HEXOSAMINE, HEXURONIC ACID, AND HYDROXYPROLINE FROM TISSUES BY RESIN HYDROLYSIS
- 1 January 1958
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry and Physiology
- Vol. 36 (1) , 413-424
- https://doi.org/10.1139/y58-047
Abstract
Excess sulphonated polystyrene resin (Dowex-50, 200–400 mesh) suspended in 0.05 N hydrochloric acid has been found suitable as a catalyst for the hydrolysis of tissue samples preparatory to the determination of hexosamine, hexuronic acid, and hydroxyproline. This technique protected hexuronic acid from destruction during hydrolysis sufficiently well to permit of its determination. The resin used in hydrolysis has been used also for separation of sugars and hexuronic acid from hexosamine and hydroxyproline by differential elution.Keywords
This publication has 4 references indexed in Scilit:
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- STUDIES ON THE GLYCOPROTEINS OF THE DOMESTIC FOWL: I. A MODIFICATION OF THE METHOD OF ELSON AND MORGAN FOR THE DETERMINATION OF HEXOSAMINE, AND ITS APPLICABILITY TO TISSUE HYDROLYZATESCanadian Journal of Chemistry, 1953
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