ELEVATED LEVELS OF INTERLEUKIN-8 AND TRANSFORMING GROWTH FACTOR-BETA IN BRONCHOALVEOLAR LAVAGE FLUID FROM PATIENTS WITH BRONCHIOLITIS OBLITERANS SYNDROME:

Publisher Website
Google Scholar
Abstract
Background. Obliterative bronchiolitis (OB), the most important long-term complication after lung transplantation, is thought to be a manifestation of chronic rejection within the airways, with the hallmarks inflammation and fibroproliferation. Methods. To characterize the inflammatory process in the context of OB we quantified tumor necrosis factor-α, interleukin (IL)-8, IL-10, and transforming growth factor (TGF)-β on the protein and mRNA level in bronchoalveolar lavage fluid samples obtained from patients with bronchiolitis obliterans syndrome (BOS) and without BOS. In addition, bronchial cells sampled by bronchial brushing were analyzed for mRNA expression. Results. In respiratory epithelial lining fluid (ELF) from BOS patients the protein levels of IL-8 (52.4±22.2 vs. 4.4±0.9 pg/ml ELF, P P P =0.11) and IL-8 (0.25±0.13 vs. 0.09±0.03 O.D./O.D. GAPDH, P =0.53) without the differences reaching statistical significance. In contrast, IL-8 mRNA expression of bronchial cells was significantly higher in the BOS group (0.85±0.40 vs. 0.22±0.10 O.D./O.D. GAPDH, P <0.05). Conclusions. We assume that IL-8 and TGF-β may act as key mediators for airway inflammation and fibroproliferation in the pathogenesis of OB, with bronchial epithelial cells serving as a relevant source of IL-8.

This publication has 27 references indexed in Scilit: