Ploidy Variation inSolanum brevidensPlants Regenerated from Protoplasts Using an Improved Culture System
- 1 February 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 37 (2) , 253-261
- https://doi.org/10.1093/jxb/37.2.253
Abstract
Plating efficiency of cultured protoplasts of Solanum brevidens increased to c. 10% after the addition of 5⋅0 mol m−3 glutamine to the culture medium. Growth of protoplast-derived colonies at densities of 20 colonies cm−3 was obtained by lowering the auxin content alter 4–6 d. A sample of 50 protoplast-derived plants was examined for chromosomal variation. Twelve plants were diploid (2n=24), 26 were tetraploid (2n=48) and 12 were aneuploid at the tetraploid level (2n=48±). Tetraploid and aneuploid plants had broader leaves and set fewer or no seeds compared to the diploid regenerants which were similar in gross morphology and seed set to control plants.Keywords
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