Studies on β-glucuronidase. 4. The purification and properties of ox-liver β-glucuronidase

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Abstract
A method was devised for the purification of ox-liver beta-glucuronidase, using reactions with metallic ions and organic solvents. An 800-fold purification was achieved by such means, with a 5% recovery of the enzyme. The pH-activity curve of the purified material shows optima at 4.5, 5.2 and 6.3, and the possible complexity of the enzyme is indicated. The isoelectric point of purified ox-liver beta-glucuronidase by filter-paper electrophoresis was found to be pH 4.85. No evidence is forthcoming from the present work to indicate that ox-liver beta-glucuronidase contains a coenzyme dissociating from the molecule on dilution. Details are given of the inhibition and activation of ox-liver beta-glucuronidase by desoxyribonucleic acid, suramin, alginic acid and a number of other organic acids, the exact effect in each case varying with pH.