Two-Stage PCR Protocol Allowing Introduction of Multiple Mutations, Deletions and Insertions Using QuikChange ^TM Site-Directed Mutagenesis

Open Access

1 April 1999

journal article
Published by Taylor & Francis in BioTechniques

Vol. 26 (4) , 680-682
https://doi.org/10.2144/99264st03

Abstract

We developed a two-stage procedure, based on the QuikChangeTM Site-Directed Mutagenesis Protocol, that significantly expands its application to a variety of gene modification experiments. A pre-PCR...

Keywords

INTRODUCTION
PROTOCOL ALLOWS
PCR
DELETIONS AND INSERTIONS
MULTIPLE MUTATIONS
QUIKCHANGE
MUTAGENESIS
PRE

Two-Stage PCR Protocol Allowing Introduction of Multiple Mutations, Deletions and Insertions Using QuikChange TM Site-Directed Mutagenesis

Abstract

Keywords

Two-Stage PCR Protocol Allowing Introduction of Multiple Mutations, Deletions and Insertions Using QuikChange ^TM Site-Directed Mutagenesis