Reduction of Zearalenone to Zearalenol in Female Rat Liver by 3α‐Hydroxysteroid Dehydrogenase

Abstract
The distribution of the zearalenone reducing activity was investigated in liver fractions obtained by differential centrifugation of liver homogenate from adult female Sprague Dawley rats. The zearalenone reducing enzyme was identified as 3α‐hydroxysteroid dehydrogenase. At least two multiple forms occur of the enzyme with different subcellular locations and pH‐optima. The activity was localized in the microsomes with NADH as coenzyme and in both microsomes and cytosol with NADPH.