Capillary Zone Electrophoretic Separation of Homovanillic and Vanillylmandelic Acids
- 1 December 1992
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 15 (18) , 3193-3201
- https://doi.org/10.1080/10826079208020878
Abstract
Capillary zone electrophoresis with untreated fused-silica capillaries and acetate buffer was evaluated for the separation and analysis of catecholamine metabolites. Homovanillic acid and vanillylmandelic acid, which are excreted in abnormally elevated levels in the urine of patients with neuroblastoma, were separated from other possible catecholamine metabolities, using a 200 mM acetate buffer at pH = 4.10. The high buffer concentration was necessary to minimize the peak tailing resulting from analyte-capillary wall interactions. The concentration detection limits of the injected sample at 214 nm were 36 mg/L for vanillylmandelic acid and 64 mg/L for homovanillic acid.Keywords
This publication has 11 references indexed in Scilit:
- Micellar Electrokinetic Capillary Chromatography: Basic Considerations and Current TrendsJournal of Liquid Chromatography, 1992
- The effect of electric field strength, buffer type and concentration on separation parameters in capillary zone electrophoresisChromatographia, 1991
- Separations by High Performance Liquid Chromatography and Capillary Zone Electrophoresis: A Comparative StudyJournal of Liquid Chromatography, 1991
- Improvement of resolution in the capillary electrophoretic separation of catecholamines by complex formation with boric acid and control of electroosmosis with a cationic surfactantJournal of Chromatography A, 1991
- Determination of urinary vanillylmandelic acid and homovanillic acid by high performance liquid chromatography for mass screening of neuroblastoma.The Tohoku Journal of Experimental Medicine, 1986
- Neuroblastoma. Mass screening for early detection and its prognosisCancer, 1984
- Zone electrophoresis in open-tubular glass capillariesAnalytical Chemistry, 1981
- Random one-hour urine catecholamine and 4-hydroxy-3-methoxymandelic acid assays for diagnosis of pheochromocytomaClinica Chimica Acta; International Journal of Clinical Chemistry, 1980
- High-performance zone electrophoresisJournal of Chromatography A, 1979
- Gas chromatographic determination of urinary vanilglycolic acid, vanilglycol, vanilacetic acid and vanillactic acid — Chemical parameters for the diagnosis of neurogenic tumours and the evaluation of their treatmentClinica Chimica Acta; International Journal of Clinical Chemistry, 1976