High‐Density culture of mouse–human hybridoma in serum‐free defined medium
- 5 February 1988
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 31 (2) , 168-172
- https://doi.org/10.1002/bit.260310210
Abstract
Mouse–human hybridoma 4H11 cells producing anti-Pseudomonas sp. monoclonal antibody (IgA) grew in a serum-free medium supplemented with insulin, transferrin, ethanolamine, and selenite (ITES). The hybridoma could be applied to high-density culture in a serum-free medium supplemented with ITES, 0.5% BSA, egg yolk VLDL, and artificial blood FC-43 in a culture vessel equipped with hollow-fiber modules for medium exchange. Total cell density reached 1.1 × 107 cells/mL (viable cell density was 7.6 × 106 cells/mL), and the IgA productivity was around 20 μg/106 cells/day in the serum-free medium, which corresponded to the levels in serum-supplemented medium.This publication has 4 references indexed in Scilit:
- Large-Scale Cell Culture TechnologyPublished by Elsevier ,1983
- Growth of hybridoma cells in serum-free medium: ethanolamine is an essential component.Proceedings of the National Academy of Sciences, 1982
- Microencapsulated Islets as Bioartificial Endocrine PancreasScience, 1980
- The cultivation of animal cells at controlled dissolved oxygen partial pressureBiotechnology & Bioengineering, 1968