Incorporation of the Enantiomers of Lipoic Acid into the Pyruvate Dehydrogenase Complex fromEscherichia coliin vivo
- 1 January 1992
- journal article
- Published by Walter de Gruyter GmbH in Biological Chemistry Hoppe-Seyler
- Vol. 373 (1) , 333-336
- https://doi.org/10.1515/bchm3.1992.373.1.333
Abstract
The uptake of 35S-labelled enantiomers of lipoic acid into cells from Escherichia coli was studied. The R-enantiomer was taken up by a factor of two more efficiently than the S-form. Autoradiography of polyacrylamide gels of partially purified pyruvate dehydrogenase complex from these cells showed that only the R-lipoic acid was covalently incorporated as a cofactor into the dihydrolipoamide acetyltransferase component of the pyruvate dehydrogenase complex.Keywords
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