Insulin receptors in rat brain: insulin stimulates phosphorylation of its receptor β‐subunit
- 25 June 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 172 (1) , 87-90
- https://doi.org/10.1016/0014-5793(84)80879-0
Abstract
In rat brain cortex synaptosomes insulin stimulated the phosphorylation of its own receptor β-subunit (94 kDa) as identified by immunoprecipitation with anti-insulin or anti-receptor antiserum. The receptor α-subunit (115 kDa) was characterized by specific labeling with 125I-labeled photoreactive insulin. These observations indicate that: (i) insulin receptors in brain are composed of α-subunits which bind insulin, and β-subunits, the phosphorylation of which can be stimulated by insulin; (ii) the size of α-subunits in brain is significantly smaller than in other tissues (115 vs 130 kDa), whereas β-subunits (94 kDa) are identical. We suggest that brain insulin receptors represent a subtype regarding their binding function, whereas their enzyme function is more conservedKeywords
This publication has 9 references indexed in Scilit:
- Structural differences between insulin receptors in the brain and peripheral target tissues.Journal of Biological Chemistry, 1983
- Receptor-mediated phosphorylation of the hepatic insulin receptor: Evidence that the M r 95,000 receptor subunit is its own kinaseProceedings of the National Academy of Sciences, 1983
- Receptors for Vasoactive Intestinal Polypeptide on Isolated Synaptosomes from Rat Cerebral Cortex. Heterogeneity of Binding and Desensitization of ReceptorsJournal of Neurochemistry, 1982
- Internalized insulin receptors are recycled to the cell surface in rat hepatocytes.Proceedings of the National Academy of Sciences, 1982
- Phosphorylation of the hepatic insulin receptorFEBS Letters, 1982
- Insulin Stimulates the Phosphorylation of the 95,000-Dalton Subunit of Its Own ReceptorScience, 1982
- Characterization of insulin receptor subunits in brain and other tissues by photoaffinity labelingBiochemical and Biophysical Research Communications, 1980
- Identification of insulin in rat brain.Proceedings of the National Academy of Sciences, 1978
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970