Evaluation of Air Samplers for Recovery of Artificially Generated Aerosols of Pure Cultures in a Controlled Environment
- 1 August 1989
- journal article
- research article
- Published by Elsevier in Journal of Food Protection
- Vol. 52 (8) , 560-563
- https://doi.org/10.4315/0362-028x-52.8.560
Abstract
All Glass Impinger-30 (AGI-30), Andersen 6-stage Sieve Air Sampler (Andersen impactor), Reuter centrifugal air sampler (RCS sampler), and Millipore open type membrane filter sampler (Filter sampler) were evaluated for recovery of artificially-generated aerosols of pure cultures of Pseudomonas fluorescens, Escherichia coli, Streptococcus faecalis, and endospores of Bacillus subtilis. Size distribution of the aerosol was analyzed to determine its relationship to air sampler efficiency. Five min after the spray, 71 to 85% of particles were between 1.1–3.3 μm in size and 3.5 to 10.4% were smaller than 1.1 μm in size. The Andersen impactor recovered significantly greater numbers of nonsporeforming bacteria, with least variability of recovery and the RCS sampler recovered significantly smaller numbers and exhibited greatest variability of the four samplers tested. AGI-30 and Filter sampler performance varied depending on the species tested. For recovery of bacterial endospore, the Filter and Andersen samplers b...This publication has 1 reference indexed in Scilit:
- Comparison of bacterial recovery by Reuter centrifugal air sampler and slit-to-agar samplerApplied and Environmental Microbiology, 1982