LOCALIZATION OF ENOLASE-1 AND ENOLASE-2 RESPECTIVELY ON CHROMOSOME-1 AND CHROMOSOME-12 USING MAN-MOUSE HYBRID ANALYSIS

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 20  (3) , 153-157
Abstract
The study of enolase in man-mouse somatic hybrids confirms synteny between ENO1 [enolase-1] and the markers on human chromosome 1 (AK2, PGM1 [phosphoglucomutase-1], Pep-C [peptidase-C]) and synteny between ENO2 and the markers on human chromosome 12 (LDHB [lactic dehydrogenase-B], Pep-B). The different enolase bands observed in mouse cell strains (Cl1D, R4, A9 neoplastic cells and 3T3 fibroblasts), in hamster cell strains (CH, V79/4, A3), and in 3 of the different bands observed in human fibroblasts have a dimeric structure. The formation of these enolase bands depends on genes at 2 different loci, .alpha. and .beta.. The hamster cell line CH (HGPRT) showed a rare enolase phenotype with a 2-banded pattern in the intermediate region, a triple-banded pattern in the slow region, and 1 single isozyme in the fast region. This hamster strain is heterozygous for the 1st locus and homozygous for the 2nd one. The relationship between these different enolase bands is as follows: in the slow ''a'' zone, .alpha.1.alpha.1,.alpha.1 .alpha.2,.alpha.2.alpha.2; in the intermediate ''i'' zone, .alpha.1.beta.1, .alpha.2.beta.1; and in the fast ''b'' zone, .beta.1.beta.1. It appears that the frequency of heterozygotes for the .alpha. or .beta. loci in man is very low. Of 32 unrelated fibroblast strains investigated, none was heterozygous for the .alpha. or .beta. locus.

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