Propagation of Trachoma Virus in Cultures of Human Tissues.

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Abstract
A strain of trachoma virus has been propagated in a synovial cell line of human origin. When coverslip preparations are stained with acridine orange fluorochrome and observed with UV, the cytoplasmic inclusion bodies appear red (ribonucleic acid-staining) in the early stages and yellow-green (deoxyribonucleic acid-staining) in the later stages of maturation. The frequency of inclusion bodies increased with virus passage. Positive infectivity tests for all passage levels were obtained in embryonated chicken eggs inoculated via the yolk sac.