Culturing Puccinia coronata on a Cell Monolayer of the Avena sativa Coleoptile
Open Access
- 1 December 1983
- journal article
- Published by Wiley in Journal of Phytopathology
- Vol. 108 (3-4) , 226-234
- https://doi.org/10.1111/j.1439-0434.1983.tb00583.x
Abstract
A culture of Puccinia coronata on an epidermal cell monolayer dissected from the Avena sativa caleoptile is described. To induce fungal development, infection structure formation had to be induced with a heat treatment. The host cells were fed with 1 % sucrose (or glucose or manitol) to sustain fungal growth. Under these conditions, normal development of hyphae, haustoria with haustorial mother cells and uredospores occurred. Uredospores had normal infectivity.Keywords
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