Hemorrhagic Enteritis: Virus Distribution and Sequential Development of Antibody in Turkeys
- 1 April 1981
- journal article
- research article
- Published by JSTOR in Avian Diseases
- Vol. 25 (2) , 444-453
- https://doi.org/10.2307/1589936
Abstract
Turkey poults were inoculated i.p. with hemorrhagic enteritis virus (HEV) at 4.5 wk of age. Antibody response and sequential development of viral antigen in various tissues were monitored. An enzyme-linked immunosorbent assay (ELISA) was developed to study antibody production; immunoperoxidase staining was used to determine sites of localization of the viral antigens in tissues. Results of ELISA and immunodiffusion tests were compared. ELISA detected antibody from day 3 post-infection (PI); gel diffusion detected antibody from day 5 PI. Peak ELISA antibody titer appeared from day 14 PI HEV antigen was detected from 2-6 days PI in the spleen, liver, intestine, kidney and bone marrow; peak titers in the spleen were on day 3 PI. Virus was not detected after day 6 PI.This publication has 0 references indexed in Scilit: