Diagnosis of Pertussis by the Fluorescent-Antibody Method

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Abstract
A PREVIOUS report1 on the identification of Bordetella pertussis in nasopharyngeal swabs by the fluorescent-antibody technic demonstrated the practicability of this method for rapid and precise diagnosis in 65 patients with pertussis. This report presents our experience with this test in a larger number of patients.Materials and MethodsAntiserum against B. pertussis was produced in rabbits and the globulin fraction conjugated with fluorescein isothiocyanate by the method previously outlined1 and tested for specificity of staining with stock cultures of pertussis, staphylococci, pneumococci, streptococci and Haemophilus influenzae. Cross-staining of staphylococci was eliminated by absorption of the antiserum with suspensions . . .

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