Leukocytosis-Promoting Factor of Bordetella pertussis III. Its Identity with Protective Antigen

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Abstract
Leukocytosis-promoting factor (LPF) was purified from the supernatant of a 5-day liquid culture of Bordetella pertussis strains Tohama and no. 134. The preparations appeared to be filamentous molecules (2 by 40 nm) and contained histamine-sensitizing and hemagglutinin activities in addition to leukocytosis-promoting activity. Active protection tests in mice demonstrated strong protective activities of 80 international protective units per mg of protein in LPF preparations of each strain toxoided by mild Formalin treatment. Passive protection tests in mice with anti-LPF sera indicated that anti-LPF is one of the protective antibodies.