Glutamine- and Asparagine-Dependent Protein Synthesis in Maturing Legume Cotyledons Culturedin vitro
- 1 June 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 30 (3) , 529-537
- https://doi.org/10.1093/jxb/30.3.529
Abstract
Legume cotyledons have been grown in sterile culture and shown to synthesize up to 16 mg protein in 5 d when supplied with either asparagine or glutamine as sole nitrogen sources. Glutamate, nitrate, and ureides also served as nitrogen sources for protein synthesis but to a lesser extent. Methionine sulphoximine and azaserine inhibited asparagine-dependent protein synthesis suggesting that ammonia is liberated from asparagine which is reassimilated via glutamine synthetase and glutamate synthase.Keywords
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