A photochemical technique for measuring lateral diffusion of spin-labeled phospholipids in membranes
- 1 October 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (10) , 4661-4663
- https://doi.org/10.1073/pnas.75.10.4661
Abstract
A technique is described for the measurement of lateral diffusion of any spin-labeled molecule in planar phospholipid multilayers, using low probe concentrations and a photochemical reaction to generate the initial concentration gradient. Features of the technique include simplicity of data analysis, versatility and applicability over a wide range of motional rates. Measurements were made on dimyristoyl phosphatidylcholine and dipalmitoyl phosphatidylcholine in the fluid phase (using a phospholipid with head-group spin label), and excellent agreement is found with recent NMR spin-echo measurements, as well as with other spin-label and fluorescence photobleaching results.This publication has 16 references indexed in Scilit:
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