Transglutaminase catalyzes differential crosslinking of small heat shock proteins and amyloid-β
- 11 September 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 576 (1-2) , 57-62
- https://doi.org/10.1016/j.febslet.2004.08.062
Abstract
Crosslinking of proteins by tissue transglutaminase (tTG) is enhanced in amyloid (Aβ) deposits characteristic of Alzheimer's disease and sporadic inclusion body myositis. Small heat shock proteins (sHsps) also occur in amyloid deposits. We here report the substrate characteristics for tTG of six sHsps. Hsp27, Hsp20 and HspB8 are both lysine- and glutamine-donors, αB-crystallin only is a lysine-donor, HspB2 a glutamine-donor, and HspB3 no substrate at all. Close interaction of proteins stimulates crosslinking efficiency as crosslinking between different sHsps only takes place within the same heteromeric complex. We also observed that αB-crystallin, Hsp27 and Hsp20 associate with Aβ in vitro, and can be readily crosslinked by tTGKeywords
This publication has 30 references indexed in Scilit:
- Importance of Tissue Transglutaminase in Repair of Extracellular Matrices and Cell Death of Dermal Fibroblasts After Exposure to a Solarium Ultraviolet A SourceJournal of Investigative Dermatology, 2003
- Transglutaminases: crosslinking enzymes with pleiotropic functionsNature Reviews Molecular Cell Biology, 2003
- Transglutaminase 2: an enigmatic enzyme with diverse functionsTrends in Biochemical Sciences, 2002
- Gliadin T Cell Epitope Selection by Tissue Transglutaminase in Celiac DiseaseJournal of Biological Chemistry, 2002
- Transglutaminase overexpression sensitizes neuronal cell lines to apoptosis by increasing mitochondrial membrane potential and cellular oxidative stressJournal of Neurochemistry, 2002
- Tissue transglutaminase: a possible role in neurodegenerative diseasesProgress in Neurobiology, 2000
- Sporadic Inclusion Body Myositis Correlates with Increased Expression and Cross-linking by Transglutaminases 1 and 2Published by Elsevier ,2000
- Differential Expression of Multiple Transglutaminases in Human BrainPublished by Elsevier ,1999
- Transglutaminase facilitates the formation of polymers of the β-amyloid peptideBrain Research, 1994
- Isolation of transglutaminase-reactive sequences from complex biological systems: a prominent lysine donor sequence in bovine lens.Proceedings of the National Academy of Sciences, 1992