CYTOMEGALO-VIRUS - DEVELOPMENT AND PROGRESSION OF CYTOPATHIC EFFECTS IN HUMAN CELL-CULTURE

  • 1 January 1980
    • journal article
    • research article
    • Vol. 42  (1) , 1-7
Abstract
Cytopathic effects of cytomegalovirus infection were studied in human cell cultures at various time intervals. Cells derived from human embryonic thyroid, skin-muscle and lung were infected with 5 different strains of cytomegalovirus at multiplicities of infection of approximately 5 plaque forming units/cell. Under these conditions, cell rounding and early cytoplasmic inclusions were first apparent at 5 h postinfection, whereas nuclear inclusions were first observed as a homogeneous eosinophilic bead at 24 h postinfection. Cytoplasmic and nuclear inclusions underwent extensive morphogenesis through 96-120 h postinfection. Development of nuclear inclusions included the formation of distinctive beadlike subunits, which increased in size from their first appearance at 48-72 h postinfection and underwent apparent contraction and breakup after 96 h postinfection. While the cytopathology induced by various cytomegalovirus strains studied was generally similar, the kinetics of their development was different and independent of both the multiplicities of infection and the source of the fibroblastic cells. Such cytomegalovirus strain-associated differences in cytopathology could result from variances in biologic characteristics of the strains studied.

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