Identification of oral yeasts by polyacrylamide gel electrophoresis
- 1 June 1991
- journal article
- Published by Wiley in Oral Microbiology and Immunology
- Vol. 6 (3) , 187-190
- https://doi.org/10.1111/j.1399-302x.1991.tb00475.x
Abstract
Protein profiles of sonicated cells for 9 species of yeasts isolated from oral samples were obtained by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE), and visualized with a silver stain. The profiles obtained on 12% acrylamide gels were distinct, and characteristic for 6 species of Candida, and Torulopsis glabrata, Yarrowia lipolytica, and Saccharomyces cerevisiae. Using this method, 79 fresh isolates of yeasts from saliva samples were identified; 58 as Candida albicans, 9 as Candida parapsilosis, 1 as Candida tropicalis, and 11 as T. glabrata. SDS‐PAGE offers a rapid, convenient alternative or adjunct to yeast identification systems based on carbohydrate assimilation tests.Keywords
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