Selective Detection of 2-Deoxyribonolactone in DNA
- 10 February 2005
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 127 (9) , 2806-2807
- https://doi.org/10.1021/ja0426185
Abstract
2-Deoxyribonolactone (L) is an oxidized abasic lesion that is produced by a variety of DNA damaging agents. It exhibits unique biological effects with respect to its proclivity to form DNA−protein cross-links and promutagenic base pairs. Recent evidence suggests that the levels of this lesion caused by oxidative stress are underestimated. We have developed a simple, selective method for detecting subpicomole amounts of L in DNA. The method takes advantage of the selective reaction of the butenolide (2) derived from β-elimination from L with a biotinylated derivative of cysteine. This method will be useful for analyzing the levels of this oxidized abasic site in DNA.Keywords
This publication has 13 references indexed in Scilit:
- Oxidative damage to DNA: formation, measurement and biochemical featuresMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 2003
- Tandem Lesions Are the Major Products Resulting from a Pyrimidine Nucleobase RadicalJournal of the American Chemical Society, 2003
- Covalent Trapping of Human DNA Polymerase β by the Oxidative DNA Lesion 2-DeoxyribonolactoneJournal of Biological Chemistry, 2002
- Efficient Near-Infrared Polymer Nanocrystal Light-Emitting DiodesScience, 2002
- DNA-damaging Enediyne CompoundsPublished by Elsevier ,1999
- Translesional Synthesis on DNA Templates Containing a Single Abasic SiteJournal of Biological Chemistry, 1997
- Theoretical study on the deoxyribose radicals formed by hydrogen abstractionJournal of the American Chemical Society, 1994
- Young formation age of a mantle plume sourceNature, 1993
- Mechanisms of bleomycin-induced DNA degradationChemical Reviews, 1987
- Nuclease activity of 1,10-phenanthroline-copper ion. Chemistry of deoxyribose oxidationJournal of the American Chemical Society, 1987