MOVEMENT OF14C‐PHOTOSYNTHATE INTO THE ROOTS OF WHEAT SEEDLINGS AND EXUDATION OF14C FROM INTACT ROOTS
Open Access
- 1 January 1970
- journal article
- Published by Wiley in New Phytologist
- Vol. 69 (1) , 37-46
- https://doi.org/10.1111/j.1469-8137.1970.tb04047.x
Abstract
SUMMARY: When 8‐day seedlings were exposed to14CO2in light, approximately 2 hours were required for14C to reach the apices of the roots. One method used to detect the progress of14C down the root was to scan the root with a radiochromatogram strip scanner. At this stage most14C in aqueous ethanol extracts of roots was present as sucrose, equally labelled in glucose and fructose. This was converted only slowly to other components of the soluble fraction so that after 12 hours almost 50% of14C was still retained in sucrose.14C exuded from intact roots into surrounding solutions was present in both volatile and non‐volatile compounds. In the non‐volatile fraction glutamine, glutamic and aspartic acids were the main14C‐amino acids. Exudation of14C was not affected by changing the inorganic composition of the collecting solution. A change in pH of collecting solution from 6.4 to 5.9 increased exudation. Exudation also increased markedly when 2,4‐dinitrophenol or potassium cyanide was present in the collecting solution.Keywords
This publication has 9 references indexed in Scilit:
- Phosphate Uptake Along Attached and Excised Wheat Roots Measured by an Automatic Scanning MethodAustralian Journal of Biological Sciences, 1967
- Dark CO2 Fixation and its Role in the Growth of Plant TissuePlant Physiology, 1966
- Plant Root Exudates and Their Influence upon Soil MicroorganismsPublished by University of California Press ,1965
- Carbon-14 Labelled Photosynthate in Wheat Root ExudatesNature, 1965
- The uptake of carbon dioxide by plant rootsPlant and Soil, 1962
- Amino acid uptake by plant rootsArchives of Biochemistry and Biophysics, 1962
- A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counterAnalytical Biochemistry, 1960
- Root excretions in relation to the rhizosphere effectPlant and Soil, 1959
- Carbon Dioxide Fixation by Barley RootsJournal of Experimental Botany, 1953