COMPARISON OF PAPER AND THIN-LAYER RADIOCHROMATOGRAPHY FOR THE QUANTITATIVE ANALYSIS OF THYROIDAL IODOAMINO ACIDS
Open Access
- 1 April 1970
- journal article
- research article
- Published by Bioscientifica in Journal of Endocrinology
- Vol. 46 (4) , 527-531
- https://doi.org/10.1677/joe.0.0460527
Abstract
SUMMARY: Paper and thin-layer radiochromatography have been applied both to the analysis of iodine-containing compounds discharged by mouse thyroid glands maintained in vitro and to their distribution in hydrolysates of the gland. The results are in close agreement. The addition of thyroid-stimulating hormone to the medium supporting the mouse thyroid glands increased the release of labelled iodothyronines. Compared with paper, thin-layer chromatography separated thyroxine and tri-iodothyronine more effectively; there was also less deiodination and a higher degree of reproducibility and reliability.Keywords
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