A monoclonal antibody against human colonic adenoma recognizes difucosylated Type-2-blood-group chains
- 1 February 1983
- journal article
- research article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 3 (2) , 163-170
- https://doi.org/10.1007/bf01121947
Abstract
A monoclonal antibody C14/1/46/10 showing preferential binding to membranes of human colorectal carcinomas over normal colon mucosae was obtained by immunization of mice with extra-nuclear membranes of a human colonic adenoma. Binding and inhibition of binding assays using blood cells or glycoproteins with known blood-group activities indicated that the antibody recognizes a carbohydrate antigen co-existing with the blood-group-H determinant: Fucα1→2Gal. Inhibition assays with structurally defined oligosaccharides showed that the antigenic determinant involves difucosylated Type-2-blood-group chains with the structure:This publication has 25 references indexed in Scilit:
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