Preparation and Immunologic Comparison of Antibodies to Closely Related Antigens Produced in Individual Rabbits
Open Access
- 1 February 1963
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 90 (2) , 318-323
- https://doi.org/10.4049/jimmunol.90.2.318
Abstract
Summary: A procedure for the isolation of two antibodies specific for very similar antigens, glucosyl-phenylazo bovine serum albumin and galactosyl-phenylazo bovine serum albumin, from doubly-immunized individual rabbits is described. Immunoelectrophoretic and ultracentrifugal analyses of these purified antibodies failed to reveal differences between them, indicating only one 7 S component for each; while total amino acid analyses and total sugar analyses also failed to reveal differences in composition. Factors which may be responsible for their difference in specificity are discussed.Keywords
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