Enhancement of erythroid colony formation in vitro by spleen extract from irradiated rats.

Publisher Website
Google Scholar
Abstract
The effect of spleen extract from irradiated rats on CFU-e [erythroid colony-forming units] and BFU-e [erythropoietic burst-forming cells] colony formation of rat bone marrow cells was investigated using modified plasma clot culture media. In the presence of erythropoietin (Ep), CFU-e colony formation peaked at 48 h of culture, and the Ep-induced increase of CFU-e colonies was dose-dependent. The addition of spleen extract enhanced colony formation more than 2-fold in the Ep-containing culture. BFU-e colony formation was also enhanced by the addition of spleen extract. Spleen extract from irradiated rats contains factor(s) which stimulates the proliferation of erythroid progenitors.